6BV0
Crystal structure of porcine aminopeptidase-N with Arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 260.646, 62.865, 81.721 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.860 |
| R-factor | 0.21725 |
| Rwork | 0.215 |
| R-free | 0.26029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fke |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 107699 | |
| <I/σ(I)> | 18.57 | 1.89 |
| Completeness [%] | 98.2 | 84.4 |
| Redundancy | 4.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 18% PEG3350, 200 MM LITHIUM SULFATE, 100 MM HEPES |
