6BUY
Crystal structure of porcine aminopeptidase-N with Glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 260.606, 62.733, 81.636 |
| Unit cell angles | 90.00, 100.26, 90.00 |
Refinement procedure
| Resolution | 128.220 - 2.100 |
| R-factor | 0.19917 |
| Rwork | 0.197 |
| R-free | 0.24804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fke |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.628 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 72167 | 7492 |
| <I/σ(I)> | 20.11 | 3.13 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 18% PEG3350, 200 MM LITHIUM SULFATE, 100 MM HEPES |
