6BUX
CRYSTAL STRUCTURE OF APOBEC3G CATALYTIC DOMAIN COMPLEX WITH SUBSTRATE SSDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.413, 47.237, 51.462 |
| Unit cell angles | 90.00, 103.13, 90.00 |
Refinement procedure
| Resolution | 38.608 - 1.856 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3IR2 (Chain A) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.762 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.856 | 1.856 |
| Number of reflections | 18860 | 1861 |
| <I/σ(I)> | 24.16 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.3 |
| CC(1/2) | 0.989 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG6000, 50 mM di-Sodium L-Malate; pH5.0, 30 mM CaCl2, Vapor Diffusion, Sitting Drop, Temperature 277K |
