6BUW

Thermus thermophilus 70S complex containing 16S G299A ram mutation and empty A site.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	210.238, 449.361, 618.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.992 - 3.500
R-factor	0.2087
Rwork	0.207
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v97
RMSD bond length	0.004
RMSD bond angle	0.817
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.12-2829)
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	121.992	121.992	3.590
High resolution limit [Å]	3.500	15.650	3.500
Rmerge	0.175	0.018	0.891
Rmeas	0.206	0.021	1.047
Total number of observations	2440921
Number of reflections	714368	8272	53336
<I/σ(I)>	8.11	39.31	1.56
Completeness [%]	97.9	94.3	99.4
Redundancy	3.417	3.365	3.492
CC(1/2)	0.995	0.999	0.356

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC