6BUH
Crystal structure of a membrane protein, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.677, 124.581, 126.712 |
| Unit cell angles | 90.00, 96.99, 90.00 |
Refinement procedure
| Resolution | 125.770 - 3.150 |
| R-factor | 0.2768 |
| Rwork | 0.276 |
| R-free | 0.29920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bug |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.770 | 3.240 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 54118 | 4222 |
| <I/σ(I)> | 8.6 | 1.2 |
| Completeness [%] | 98.5 | 87.8 |
| Redundancy | 3.6 | 2.9 |
| CC(1/2) | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100mM sodium citrate pH5.6, 200mM NH4H2PO4, and 100mM (NH4)2SO4, 27% (w/v) PEG 400 |
