6BUG
Crystal structure of a membrane protein, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.713, 121.119, 126.529 |
| Unit cell angles | 90.00, 101.58, 90.00 |
Refinement procedure
| Resolution | 123.950 - 3.270 |
| R-factor | 0.2897 |
| Rwork | 0.289 |
| R-free | 0.31090 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.950 | 3.400 |
| High resolution limit [Å] | 3.270 | 3.300 |
| Number of reflections | 46614 | 4072 |
| <I/σ(I)> | 13.1 | 1.2 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 6.4 | 6.3 |
| CC(1/2) | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100mM Tris-HCl pH7.5, 100mM NaCl, 100mM MgCl2, 21% (w/v) PEG 400 |
