6BTX
Structure of a bacterial metal transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.072, 54.109, 72.442 |
Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
Resolution | 42.904 - 3.200 |
R-factor | 0.2264 |
Rwork | 0.223 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.521 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.904 | 3.314 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 6989 | |
<I/σ(I)> | 6.29 | |
Completeness [%] | 96.2 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100 mM Tris pH 8.0, 100 mM Na-acetate, 70 mM CaCl2, and 20% PEG500DME |