6BTJ

Crystal structure of pan-H7, anti-hemagglutinin monoclonal antibody H7.5 (Fab fragment)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	77.2
Detector technology	PIXEL
Collection date	2016-03-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 2 21 21
Unit cell lengths	65.510, 99.038, 208.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.519 - 1.999
R-factor	0.1998
Rwork	0.200
R-free	0.20110
RMSD bond length	0.009
RMSD bond angle	1.138
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PARROT
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmeas	0.123	0.810
Rpim	0.047	0.320
Number of reflections	91327	4421
<I/σ(I)>	21	1.5
Completeness [%]	98.5	96.6
Redundancy	6.8	6.3
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1M MES pH6.5 0.01M cobalt chloride 1.8M ammonium sulfate