6BTF
DNA Polymerase Beta I260Q Ternary Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.610, 79.879, 55.382 |
| Unit cell angles | 90.00, 107.40, 90.00 |
Refinement procedure
| Resolution | 37.551 - 1.750 |
| R-factor | 0.1788 |
| Rwork | 0.175 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | SAINT (v2016.9-0) |
| Data scaling software | SADABS (v2016.9-0) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (v1.9-1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.098 | 0.539 |
| Rpim | 0.053 | 0.301 |
| Number of reflections | 82376 | 8164 |
| <I/σ(I)> | 12 | 3.4 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.6 | 3.3 |
| CC(1/2) | 0.992 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 18-20% PEG 3350, 175-250 mM sodium acetate, 50 mM HEPES pH 7.5, 2 mM TCEP, 1% tert-butanol |
