6BTC
SCCmec type IV LP1413 - nucleic acids binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 97.033, 97.033, 127.794 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.517 - 2.177 |
R-factor | 0.223309918529 |
Rwork | 0.220 |
R-free | 0.25119 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.661 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.517 | 2.236 |
High resolution limit [Å] | 2.177 | 2.177 |
Number of reflections | 18476 | |
<I/σ(I)> | 27.3 | |
Completeness [%] | 96.8 | |
Redundancy | 27.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | 100mM NaCitrate, pH6, 10mM MgCl2-1.8M Li2SO4-14% Glycerol-5mM DTT |