6BSZ
Human mGlu8 Receptor complexed with glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-05 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 112.539, 166.623, 198.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 2.650 |
R-factor | 0.2516 |
Rwork | 0.250 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | rat mGlu7 (rmGlu) ATD (PDB: 2D4Z) |
RMSD bond length | 0.005 |
RMSD bond angle | 0.790 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.940 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 51038 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 98.9 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100 mM sodium citrate pH 6, 1.6 M ammonium sulfate, 0.2 M sodium potassium tartrate, and 5 mM L-glutamate. |