6BSE

Glucocorticoid receptor bound to high cooperativity monomer sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-10-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	39.447, 95.172, 104.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.750 - 2.350
R-factor	0.194
Rwork	0.192
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g99
RMSD bond length	0.012
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.710	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.050	0.325
Rmeas	0.070	0.459
Rpim	0.050	0.325
Number of reflections	17036	1678
<I/σ(I)>	15.25	2.47
Completeness [%]	98.7	99.88
Redundancy	2	2
CC(1/2)	0.995	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.08 M KCl 0.04 M Sodium cacodylate trihydrate 60% v/v (+/-)-2-Methyl-2,4-pentanediol 0.012 M Spermine tetrahydrochloride