6BRP

F-box protein form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	79.451, 130.471, 94.316
Unit cell angles	90.00, 99.38, 90.00

Refinement procedure

Resolution	93.060 - 2.390
Rwork	0.192
R-free	0.21800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.060	2.478
High resolution limit [Å]	2.390	2.392
Number of reflections	73476	7246
<I/σ(I)>	26.4
Completeness [%]	98.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	80 mM Tris-HCl, pH 7.0, 24% MPD, 24% PEG1000, 24% P3350; 15 mM Sodium Citrate tribasic dihydrate pH 5.6; 0.5 M 1,6-Hexanediol