6BRO

Crystal structure of ASK1-D3 ubiquitin ligase form1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	233.728, 79.730, 153.437
Unit cell angles	90.00, 128.59, 90.00

Refinement procedure

Resolution	119.930 - 2.500
R-factor	0.2034
Rwork	0.202
R-free	0.23360
RMSD bond length	0.010
RMSD bond angle	1.499
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.930	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	75576
<I/σ(I)>	35.5
Completeness [%]	98.8
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	6.5% CP-42; 175 mM Sodium Citrate tribasic dihydrate; 87 mM HEPES sodium pH 7.5; 26% MPD