6BRL
Crystal structure of a glutamate tRNA ligase from Elizabethkingia meningosepticum CCUG26117 in complex with its amino acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.260, 111.890, 130.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.854 | 35.854 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.063 | 0.029 | 0.572 |
Rmeas | 0.069 | 0.032 | 0.624 |
Number of reflections | 43563 | 552 | 3169 |
<I/σ(I)> | 17.75 | 40.94 | 3.21 |
Completeness [%] | 99.8 | 95.7 | 99.9 |
Redundancy | 6.092 | 4.971 | 6.175 |
CC(1/2) | 0.999 | 0.999 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | MCSG1 E10 (295949e10): 200mM Ammonium tartarate dibasic, 20% (w/v) PEG3350: ElmeA.01348.a.B1.PW38364 16.23 mg/mL, direct cryo: xxk4-10 |