6BRD
Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complexed with rifampin and FAD
Replaces: 5VQCExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-04-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.026, 129.402, 74.342 |
| Unit cell angles | 90.00, 105.45, 90.00 |
Refinement procedure
| Resolution | 33.417 - 3.320 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kow |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.417 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 27436 | |
| <I/σ(I)> | 5.55 | 1.33 |
| Completeness [%] | 97.1 | 83.3 |
| Redundancy | 5.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M sodium cacodylate, pH 6.5, 20% w/v PEG8000, 0.2 M magnesium acetate, soaked in rifamycin |
