6BR9
Structure of A6 reveals a novel lipid transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-18 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.699, 74.151, 143.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 89447 | |
| <I/σ(I)> | 18.5 | 2.6 |
| Completeness [%] | 99.3 | 95.5 |
| Redundancy | 5.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium thiocyanate, 25% PEG3350 |
