6BR8

Structure of A6 reveals a novel lipid transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-18
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97935
Spacegroup name	P 21 21 21
Unit cell lengths	54.909, 75.163, 148.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.338 - 2.300
R-factor	0.2326
Rwork	0.228
R-free	0.28910
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.223
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	50967	2648
<I/σ(I)>	21.6	1.2
Completeness [%]	98.6	96.2
Redundancy	5.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M sodium thiocyanate, 25% PEG3350