6BQU

Human GR (418-507) in complex with monomeric DNA binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-19
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	38.231, 88.235, 103.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.952 - 2.500
R-factor	0.215
Rwork	0.213
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g99
RMSD bond length	0.005
RMSD bond angle	0.687
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.952	2.750
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.836
Number of reflections	12750	3122
<I/σ(I)>	16.2
Completeness [%]	99.7	100
Redundancy	6.8	6.9
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.005 M Magnesium sulfate heptahydrate, 0.05 M MES monohydrate, pH 6.0, 5 percent w/v, Polyethylene glycol 4,000