6BPM
The crystal structure of the Ferric-Catecholate import receptor Fiu from K12 E. coli: Closed form (C21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.432, 75.668, 136.870 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 49.345 - 2.500 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fp1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.560 |
| Rmerge | 0.156 | 1.026 |
| Rpim | 0.147 | 0.950 |
| Number of reflections | 68066 | 4585 |
| <I/σ(I)> | 5.4 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.988 | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 3350, 4% Polypropylene Glycol P400, 0.2 M NaCl, 0.1 M Tris |
