6BPH

Crystal structure of the chromodomain of RBBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-18
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	41.493, 41.493, 81.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.900 - 1.850
R-factor	0.207
Rwork	0.206
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lcc
RMSD bond length	0.010
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.970	36.970	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.072	0.026	1.077
Rmeas	0.075	0.027	1.119
Rpim	0.020	0.009	0.300
Total number of observations	92703	728	5174
Number of reflections	7057
<I/σ(I)>	26.2	69.7	3.3
Completeness [%]	99.6	99.8	98.8
Redundancy	13.1	8.7	13.3
CC(1/2)	1.000	1.000	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% PEG 3350, 0.2M magnesium chloride, 0.1M HEPES, pH 7.5