6BPE
Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to monoclonal antibody 6H1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.600, 123.480, 143.280 |
Unit cell angles | 90.00, 105.25, 90.00 |
Refinement procedure
Resolution | 73.746 - 3.340 |
R-factor | 0.3186 |
Rwork | 0.318 |
R-free | 0.36670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w53 5en2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.720 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.2.7) |
Phasing software | PHENIX (1.12_2829) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.490 | 3.440 |
High resolution limit [Å] | 3.340 | 3.340 |
Number of reflections | 51887 | 4485 |
<I/σ(I)> | 5.5 | 1.2 |
Completeness [%] | 99.5 | 99.2 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 0.992 | 0.612 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M (NH4)2SO4, 20% (w/v) PEG 3,350 |