6BPC

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to monoclonal antibody 4F7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	70.090, 70.310, 140.363
Unit cell angles	104.18, 100.34, 98.41

Refinement procedure

Resolution	66.308 - 2.660
R-factor	0.1996
Rwork	0.197
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53 5en2
RMSD bond length	0.005
RMSD bond angle	0.675
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.2.7)
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.550	2.720
High resolution limit [Å]	2.660	2.660
Number of reflections	67863	4417
<I/σ(I)>	6	1.9
Completeness [%]	94.3	94.3
Redundancy	3.6	3.5
CC(1/2)	0.982	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M trisodium citrate and 20% (w/v) PEG 3,350