6BP6
Crystal structure of Commd9 COMM domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9787 |
Spacegroup name | I 4 |
Unit cell lengths | 79.404, 79.404, 58.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.366 - 2.170 |
R-factor | 0.2514 |
Rwork | 0.248 |
R-free | 0.28550 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.637 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.13rc1_2954: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.130 | 2.240 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.060 | |
Number of reflections | 9689 | |
<I/σ(I)> | 26.7 | |
Completeness [%] | 99.7 | |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M HEPES (pH 7.0), 6% Jeffamine M-600 |