6BOT
Human APE1 substrate complex with an C/C mismatch adjacent the THF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.247, 60.944, 73.292 |
| Unit cell angles | 83.18, 78.48, 87.21 |
Refinement procedure
| Resolution | 48.910 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.320 |
| Rmerge | 0.082 | 0.573 |
| Number of reflections | 62321 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 7% PEG 20K, 100mM sodium Citrate, 15% glycerol, 5mM CaCl2 |
