6BNU
Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | FEI TECNAI 20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 7.5 |
Software | Appion,EMAN2,SPARX,FREALIGN,PHENIX,Leginon,CTFFIND |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |