6BNU
Structure of bare actin filament, backbone-averaged with sidechains truncated to alanine
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 7.5 |
| Software | Appion,EMAN2,SPARX,FREALIGN,PHENIX,Leginon,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
