6BNP
CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.6 |
| Software | Appion,NAMD,MDFF,EMAN2,SPARX,FREALIGN,Leginon,CTFFIND,DireX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
