6BNN
Crystal structure of V278E-glyoxalase I mutant from Zea mays in space group P4(1)2(1)2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.950, 69.950, 119.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.700 - 1.550 |
R-factor | 0.19097 |
Rwork | 0.189 |
R-free | 0.23152 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d7z |
RMSD bond length | 0.017 |
RMSD bond angle | 1.812 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.700 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmeas | 0.065 | 1.653 |
Number of reflections | 43782 | 6880 |
<I/σ(I)> | 19.3 | 1.4 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 12.9 | 13.2 |
CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.7 | 294 | sodium formate 4.2 M pH 9.7 0.5% PEG 4000 |