6BNA
BINDING OF AN ANTITUMOR DRUG TO DNA. NETROPSIN AND C-G-C-G-A-A-T-T-BRC-G-C-G
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.270, 39.620, 63.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.210 |
| R-factor | 0.211 |
| Refinement software | JACK-LEVITT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 3240 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 * | VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | SPERMINE_HCL | ||
| 4 | 1 | 1 | MG ACETATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.30 (mM) | |
| 2 | 1 | drop | netropsin | 0.60 (mM) | |
| 3 | 1 | drop | spermine hydrochloride | 0.30 (mM) | |
| 4 | 1 | drop | magnesium acetate | 6.30 (mM) | |
| 5 | 1 | drop | MPD | 10 (%(v/v)) | |
| 6 | 1 | reservoir | 32.5 (%) |
