6BN1

Salvador Hippo SARAH domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 2 2
Unit cell lengths	49.934, 49.934, 186.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.903 - 2.600
R-factor	0.2042
Rwork	0.202
R-free	0.24590
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.903	2.900
High resolution limit [Å]	2.600	2.600
Number of reflections	7868
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	24.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM Sodium Cacadylate 35% MPD 2% PEG 20K