6BN0
Avirulence protein 4 (Avr4) from Cladosporium fulvum bound to the hexasaccharide of chitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.11583 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.864, 41.081, 121.365 |
Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
Resolution | 60.111 - 1.950 |
R-factor | 0.1698 |
Rwork | 0.168 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z4a |
RMSD bond length | 0.013 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.111 | 60.111 | 2.000 |
High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
Rmerge | 0.054 | 0.023 | 0.612 |
Rmeas | 0.063 | 0.027 | 0.716 |
Number of reflections | 28654 | 360 | 2110 |
<I/σ(I)> | 13.69 | 38.19 | 2.12 |
Completeness [%] | 99.6 | 98.4 | 99.8 |
Redundancy | 3.646 | 3.569 | 3.72 |
CC(1/2) | 0.999 | 0.999 | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris:HCl, pH 8.5, 30% PEG 4000, 0.8 M LiCl |