6BMH
Crystal structure of MHC-I like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.955, 74.229, 117.594 |
| Unit cell angles | 90.00, 102.94, 90.00 |
Refinement procedure
| Resolution | 45.931 - 2.298 |
| R-factor | 0.2323 |
| Rwork | 0.230 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cd1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.043 | |
| Number of reflections | 79496 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 100 mM BisTris (pH 6-6.5), 200 mM CaCl2, 21-26% PEG 3350 |
