6BME

Crystal structure of Chlamydomonas reinhardtii THB4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	110
Detector technology	CCD
Collection date	2017-06-27
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	74.400, 68.300, 49.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.677 - 1.899
R-factor	0.1915
Rwork	0.188
R-free	0.22690
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.736
Data reduction software	CrysalisPro (1.171.36.32)
Data scaling software	CrysalisPro (1.171.36.32)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.680	1.970
High resolution limit [Å]	1.899	1.899
Rmerge	0.028	0.169
Number of reflections	38306	3832
<I/σ(I)>	32.37	5
Completeness [%]	99.9	100
Redundancy	8.5	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.2 M Ammonium Sulfate, 0.1 M Bis-Tris pH 5.5, 20% PEG 3350