6BMC

The structure of a dimeric type II DAH7PS associated with pyocyanin biosynthesis in Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95363
Spacegroup name	C 2 2 21
Unit cell lengths	53.757, 169.722, 170.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.270 - 2.700
R-factor	0.24961
Rwork	0.248
R-free	0.28978
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uxm
RMSD bond length	0.005
RMSD bond angle	0.926
Data reduction software	autoXDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.270	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.128	2.619
Rpim	0.064	1.280
Number of reflections	21959	2879
<I/σ(I)>	10.6	0.8
Completeness [%]	99.9	100
Redundancy	10	10
CC(1/2)	0.999	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.1-0.4 M NaF, 1 mM CoCl2, 1 mM PE, 16-22 % PEG 3350