6BMC
The structure of a dimeric type II DAH7PS associated with pyocyanin biosynthesis in Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95363 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.757, 169.722, 170.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.270 - 2.700 |
| R-factor | 0.24961 |
| Rwork | 0.248 |
| R-free | 0.28978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.926 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.270 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.128 | 2.619 |
| Rpim | 0.064 | 1.280 |
| Number of reflections | 21959 | 2879 |
| <I/σ(I)> | 10.6 | 0.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 | 10 |
| CC(1/2) | 0.999 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1-0.4 M NaF, 1 mM CoCl2, 1 mM PE, 16-22 % PEG 3350 |
