6BM8
Crystal structure of glycoprotein B from Herpes Simplex Virus type I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 118.597, 118.597, 800.576 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.870 - 4.100 |
| R-factor | 0.2703 |
| Rwork | 0.269 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gum |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 101.870 | 101.870 | 4.370 |
| High resolution limit [Å] | 3.990 | 9.770 | 3.990 |
| Rmerge | 0.109 | 0.026 | 1.221 |
| Rmeas | 0.131 | 0.032 | 1.460 |
| Rpim | 0.072 | 0.019 | 0.790 |
| Total number of observations | 62145 | ||
| Number of reflections | 19003 | 1392 | 4477 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.0 | 96.9 | 99.1 |
| Redundancy | 3.3 | 2.9 | 3.3 |
| CC(1/2) | 0.999 | 0.999 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 11% PEG 4000, 0.1 M Tris HCl pH 7.5, 0.1 M NaCl |
