Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977408 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.814, 67.229, 94.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.695 - 1.488 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.21110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5unq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.695 | 1.540 |
High resolution limit [Å] | 1.488 | 1.490 |
Number of reflections | 50550 | |
<I/σ(I)> | 25.49 | |
Completeness [%] | 99.7 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 33% PEG 8000 0.1 M HEPES pH 7.5 |