6BLH
RSV G central conserved region bound to Fab CB017.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.069, 189.646, 74.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.873 - 2.000 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8fab 4hpy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.523 |
| Data reduction software | MOSFLM (7.2.1) |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (7.0.033) |
| Refinement software | PHENIX (1.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40222 | 2939 |
| <I/σ(I)> | 5.3 | 1.8 |
| Completeness [%] | 98.5 | 98.7 |
| Redundancy | 5.8 | 6.1 |
| CC(1/2) | 0.988 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23.7% Isopropanol, 11.85% PEG4k, 0.1M HEPES pH 7.5 |
