6BLB
1.88 Angstrom Resolution Crystal Structure Holliday Junction ATP-dependent DNA Helicase (RuvB) from Pseudomonas aeruginosa in Complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 65 |
Unit cell lengths | 85.537, 85.537, 77.103 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.640 - 1.880 |
R-factor | 0.17452 |
Rwork | 0.172 |
R-free | 0.21307 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1in4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.369 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.910 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.076 | 0.797 |
Rmeas | 0.081 | 0.849 |
Rpim | 0.028 | 0.291 |
Number of reflections | 26154 | 1311 |
<I/σ(I)> | 26.9 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.4 | 8.3 |
CC(1/2) | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 8.6 mg/mL protein in 0.5 M sodium chloride, 0.01 M Tris, pH 8.3, screen: Classics II (D3), 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine ED-2001 |