6BLB

1.88 Angstrom Resolution Crystal Structure Holliday Junction ATP-dependent DNA Helicase (RuvB) from Pseudomonas aeruginosa in Complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 65
Unit cell lengths	85.537, 85.537, 77.103
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.640 - 1.880
R-factor	0.17452
Rwork	0.172
R-free	0.21307
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1in4
RMSD bond length	0.009
RMSD bond angle	1.369
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.910
High resolution limit [Å]	1.880	1.880
Rmerge	0.076	0.797
Rmeas	0.081	0.849
Rpim	0.028	0.291
Number of reflections	26154	1311
<I/σ(I)>	26.9	2.9
Completeness [%]	100.0	100
Redundancy	8.4	8.3
CC(1/2)		0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	8.6 mg/mL protein in 0.5 M sodium chloride, 0.01 M Tris, pH 8.3, screen: Classics II (D3), 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine ED-2001