6BL7
S. cerevisiae stu2 coiled coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-31 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97921 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.252, 30.112, 72.856 |
Unit cell angles | 90.00, 102.08, 90.00 |
Refinement procedure
Resolution | 49.317 - 2.500 |
R-factor | 0.2603 |
Rwork | 0.257 |
R-free | 0.29200 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.720 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 9566 | 1530 |
<I/σ(I)> | 18.3 | 2.3 |
Completeness [%] | 99.0 | 92.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 293 | 13% w/v PEG8000, 200 mM magnesium acetate, 100 mM sodium cacodylate, pH 5.75 |