6BL5
Head decoration protein from the hyperthermophilic phage P74-26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-21 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 |
Unit cell lengths | 89.516, 89.516, 31.390 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.841 - 1.690 |
R-factor | 0.1821 |
Rwork | 0.179 |
R-free | 0.21260 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.046 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 (v703x) |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.841 | 1.740 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.057 | 0.824 |
Rmeas | 0.062 | 0.888 |
Rpim | 0.023 | 0.328 |
Number of reflections | 16355 | 1344 |
<I/σ(I)> | 14.3 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.2 |
CC(1/2) | 0.999 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Tris-HCl pH 7.0, 20% PEG 3350 |