6BKP

Crystal structure of the A/Michigan/15/2014 (H3N2) influenza virus hemagglutinin apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	H 3 2
Unit cell lengths	100.326, 100.326, 394.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.050
R-factor	0.19339
Rwork	0.192
R-free	0.22183
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o5n
RMSD bond length	0.010
RMSD bond angle	1.476
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rpim	0.040	0.240
Number of reflections	48747	4806
<I/σ(I)>	44.3	3.1
Completeness [%]	100.0	100
Redundancy	17.4	16
CC(1/2)	1.000	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Tris pH 8.5, 0.2 M lithium sulfate, and 40% PEG 300