6BKN
Crystal structure of the A/Wyoming/3/2003 (H3N2) influenza virus hemagglutinin apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.334, 100.333, 385.597 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.16766 |
| Rwork | 0.167 |
| R-free | 0.18477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.020 | 0.240 |
| Number of reflections | 63929 | 5808 |
| <I/σ(I)> | 43.3 | 2.7 |
| Completeness [%] | 98.7 | 99.5 |
| Redundancy | 17.5 | 16 |
| CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1 M bicine pH 9 and 42% 2-methyl-2,4-pentanediol |
