6BK7

1.83 Angstrom Resolution Crystal Structure of N-terminal Fragment (residues 1-404) of Elongation Factor G from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	61.450, 86.425, 75.442
Unit cell angles	90.00, 90.17, 90.00

Refinement procedure

Resolution	28.950 - 1.830
R-factor	0.19278
Rwork	0.191
R-free	0.21734
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vh6
RMSD bond length	0.008
RMSD bond angle	1.371
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.830	1.830
Rmerge	0.046	0.793
Rmeas	0.052	0.900
Rpim	0.025	0.423
Number of reflections	69321	3419
<I/σ(I)>	28.8	2.05
Completeness [%]	99.8	99.5
Redundancy	4.5	4.4
CC(1/2)		0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	295	12.5 mg/mL protein in 0.5 sodium chloride, 0.01 M Tris, pH 8.3 against screen PEGs II (G6), 15% w/v PEG6000, 5% w/v glycerol, cryoprotectant: reservoir