6BK6
Crystal structure of Hendra virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2017-03-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953735 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.380, 60.380, 364.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.291 - 2.500 |
| R-factor | 0.2216 |
| Rwork | 0.220 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gio |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.260 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.291 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.425 | |
| Number of reflections | 14447 | |
| <I/σ(I)> | 11.2 | 3.9 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ca-Acetate, 0.1 M imadazole pH 7.5, 5% PEG 8000 |
