6BK5

Inactive choanoflagellate E3 ubiquitin ligase Cbl TKB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999996
Spacegroup name	P 31 2 1
Unit cell lengths	115.251, 115.251, 68.777
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.392 - 2.401
R-factor	0.1688
Rwork	0.167
R-free	0.20850
RMSD bond length	0.007
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	2.540
High resolution limit [Å]	2.400	2.401
Rmeas	0.075	0.527
Number of reflections	20844	5377
<I/σ(I)>	9.67	1.73
Completeness [%]	99.8	83.9
Redundancy	2.04	2.03
CC(1/2)	0.996	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2% (w/v) Tacsimate pH 7.0, 10% (w/v) polyethylene glycol 3,350