6BK4

Crystal structure of the HR-1 domain of Drosophila caprin in the P212121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	53.050, 60.040, 99.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.270 - 1.800
R-factor	0.2167
Rwork	0.215
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wbe
RMSD bond length	0.006
RMSD bond angle	0.687
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.270	60.040	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge		0.037	0.495
Rmeas	0.069	0.043	0.552
Rpim	0.030	0.020	0.240
Total number of observations	153362
Number of reflections	30126	990	4358
<I/σ(I)>	13.9	13.3	1.6
Completeness [%]	99.8	92.7	100
Redundancy	5.1	4.3	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	18% PEG2000, 0.1 M BisTris (pH6.8), 5% glycerol