6BK3
Crystal structure of Os79 from O. sativa in complex with UDP and deoxynivalenol-3-glucoside (glucose moitey not resolved)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.504, 104.504, 98.261 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.140 - 2.170 |
| R-factor | 0.19051 |
| Rwork | 0.189 |
| R-free | 0.22792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tme |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.221 |
| Data scaling software | HKL-3000 |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.170 |
| Rmerge | 0.086 |
| Number of reflections | 33082 |
| <I/σ(I)> | 29.1 |
| Completeness [%] | 100.0 |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100 mM sodium acetate pH 5.0, 40% pentaerythritol propoxylate 426, 320 mM NaCl |
