6BK2
Crystal structure of Os79 H122A/L123A from O. sativa in complex with UDP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-10 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.552, 83.118, 99.384 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.680 |
R-factor | 0.15364 |
Rwork | 0.152 |
R-free | 0.18715 |
RMSD bond length | 0.033 |
RMSD bond angle | 2.472 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.290 |
Number of reflections | 115974 |
<I/σ(I)> | 34.9 |
Completeness [%] | 99.2 |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 50 mM HEPES pH 7.0, 18% methyl ether polyethylene glycol 2000 |