6BK2
Crystal structure of Os79 H122A/L123A from O. sativa in complex with UDP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-10 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.552, 83.118, 99.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.680 |
| R-factor | 0.15364 |
| Rwork | 0.152 |
| R-free | 0.18715 |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.472 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.290 |
| Number of reflections | 115974 |
| <I/σ(I)> | 34.9 |
| Completeness [%] | 99.2 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 50 mM HEPES pH 7.0, 18% methyl ether polyethylene glycol 2000 |
