6BK0

Crystal structure of Os79 Q202A from O. sativa in complex with UDP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	59.409, 83.233, 99.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.460 - 1.470
R-factor	0.15408
Rwork	0.153
R-free	0.18068
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tme
RMSD bond length	0.041
RMSD bond angle	2.874
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.470
Number of reflections	79234
<I/σ(I)>	41.3
Completeness [%]	99.2
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	100 mM 3-(N- Morpholino)propanesulfonic acid pH 7.5, 15% methyl ether polyethylene glycol 5000 (MEPEG 5K), 160 mM potassium glutamate