6BJW
Eubacterium eligens Beta-glucuronidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 179.775, 179.775, 134.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.432 - 3.000 |
| R-factor | 0.2266 |
| Rwork | 0.224 |
| R-free | 0.25700 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.655 |
| Data reduction software | XDS |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.440 | 3.180 |
| High resolution limit [Å] | 2.856 | 3.000 |
| Rmerge | 0.111 | 0.591 |
| Rmeas | 0.116 | 0.618 |
| Rpim | 0.033 | 0.176 |
| Number of reflections | 26230 | 4133 |
| <I/σ(I)> | 20 | 5.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.3 | 12.7 |
| CC(1/2) | 0.998 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12% PEG 400 and Bis-tris pH 6.5 |
